Color catalytic tests are very sensitive, but not specific. Moisten a swab with water and touch it to a dried blood sample. Crit Rev Anal Chem. This test is a presumptive test for the presence of acid phosphatase only. doi: 10.1016/j.fct.2008.02.008. High hemoglobin levels are usually caused by an extended period of low oxygen in the blood. endstream endobj 116 0 obj<>/Metadata 17 0 R/PieceInfo<>>>/Pages 16 0 R/PageLayout/OneColumn/OCProperties<>/StructTreeRoot 19 0 R/Type/Catalog/LastModified(D:20060626150122)/PageLabels 14 0 R>> endobj 117 0 obj<>/PageElement<>>>/Name(HeaderFooter)/Type/OCG>> endobj 118 0 obj<>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC]/ExtGState<>>>/Type/Page>> endobj 119 0 obj<> endobj 120 0 obj[/ICCBased 127 0 R] endobj 121 0 obj<> endobj 122 0 obj<> endobj 123 0 obj<>stream We use operational excellence to deliver solutions that enable research, testing, production, and commercialization across the globe. 0000021854 00000 n Paper envelopes, paper sacks, or cardboard boxes should be used after allowing evidence to dry. Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials. red/brown) then there is another test called the ABA Hematrace test that can easily and rapidly determine whether or not the presumptive blood is of human origin. Understanding the discipline, its underlying scientific basis, and how best to apply 385476213573 There can be several variations of this, for example in determining a person's blood type, one may code type A whereas the other my code type B. 115 0 obj <> endobj 2023 FORTUNE Media IP Limited All rights reserved. What is a false positive test with Hemastix? Freeze cells in tubes from 1 to 5mL using the Thermo Scientific Mr. Frosty Freezing Container at nearly -1C/minute. Get a criminal history record expungement? The reagent pad will turn green or blue-green to indicate a positive test. In addition to the full CBC, other tests may be performed at the same time or as a follow-up to help with a diagnosis, including: Once your blood is collected, its placed in a centrifuge or an automated hematology analyzer instrument. red/brown) then there is another test called the ABA Hematrace test that can easily and rapidly determine whether or not the presumptive blood is . The test works regardless of the blood being human or animal. [3][4] In 1906, Kastle and Amoss found that chick hemoglobin in blood triggered the reaction. What color does the Hemastix test turn when positive? The reagent coated tip will turn a shade of green if hemoglobin is detected. 0 This is a crystalline test that is viewed using a microscope. Add a drop or two of 70 percent ethanol to the swab. BLUTFLECKENMUSTERANALYSE: MIT EINER Einfhrung in die Tatortrekonstruktion - EUR 156,24. Anemia: Cancer Treatment Side Effect. Dependable -80C storage with a focus on sustainability without sacrificing sample protection. The aim of this study was to validate the two reagents for use with animal blood, and compare their performance in order to choose the best test based on the circumstances in wildlife crime investigation. How can you tell if a blood test is a presumptive test? (b) Assume the wire is aluminum and is under a tension T = 24.0 N. Determine the wave speed at the origin and at x = 10.0 m. The sequence of nucleotides on DNA that constitutes the form of a gene at a specific spot or a chromosome. endstream endobj 124 0 obj<> endobj 125 0 obj<> endobj 126 0 obj<> endobj 127 0 obj<>stream She was diagnosed with ulcerative colitis at age 16. It is typically less sensitive than a presumptive test but more specific. If you have too many or too few red blood cells, it may be a sign of dehydration, anemia, a vitamin or mineral deficiency, recent or long-term blood loss, or a disease like leukemia, lymphoma, or polycythemia vera, among other things. The notes inlcude the four components of blood, the four blood types (phenotypes and genotypes), antigens, antibodies, agglutination, blood transfusions and universal donors. If the test result is negative, it is reasonable proof that heme (an ingredient in all blood) is absent in the sample. q0a2`z+rUZ9x/_Ow|>pCOkKu&NOyWp%amGRt9ptD>=^xa]QsnZ%fZ[*D'Z9,F3ifs }8 @ ] For further details on the history of the KastleMeyer test, see: Robert E. Gaensslen, "Phenolphthalin as a reagent for the oxidizing ferments,", "Beitrge zur Leukocytenfrage. Hemastix Test Hydrate Q-tip with distilled water (if dry) and roll onto stain Transfer Q-tip to . 0000002260 00000 n By clicking Accept All Cookies, you agree to the storing of cookies on your device to enhance site navigation, analyze site usage, and assist in our marketing efforts. 2. Disclaimer. -Test results may provide information on urinary tract status. Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for We found alternative products that can save you up to per item-unit. An HCT test is usually ordered as part of a complete blood count (CBC) in which multiple tests are run from blood taken at one time. 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A positive reaction gives a pink color. Learn more. x- [ 0}y)7ta>jT7@t`q2&6ZL?_yxg)zLU*uSkSeO4?c. R -25 S>Vd`rn~Y&+`;A4 A9 =-tl`;~p Gp| [`L` "AYA+Cb(R, *T2B- On the other hand, Hemastix showed a much lower specificity, producing false positive results when exposed to tomato, potato, rust, avian uric acid, bleach and sink rot, while Kastle Meyer only produced a faint positive reaction from potato. This extremely visual PowerPoint with tons of pictures & GIFs goes over serology for forensics or criminal investigations. A positive result will be indicated by the presence of two red lines forming on the cassette detection strip. Update to Avantors response to the coronavirus (COVID-19) pandemic. How is savings calculated?We ZU VERKAUFEN! VWR will support you from the latest life science products to the guaranteed purity of organic building blocks VWR is ready to support your production facility with reliable access to raw materials and essential supplies. North Central Investigative Regional Office, South Central Investigative Regional Office - HQ, AFIS - Automated Fingerprint Identification System, SIBRS - State Incident-Based Reporting System, NIBRS - National Incident-Based Reporting System, Sexual Assault Kit Tracking System Information, Order Sexual Assault Evidence Collection Kits, Order Fingerprint Cards - Law Enforcement Only. Commercial Distribution End Date: NA. 5 year bumper to bumper warranty! 115 18 The former two depend only upon the test, but latter two depend on the test and also the prevalence of the condition in the population. An official website of the United States government. Acid phosphatase (AP) is a class of enzymes that can catalyze the hydrolysis of certain organic phosphates. Catalog No. Donating electrons to hemoglobin converts the phenolphthalin back into the intensely colored phenolphthalein. The Kastle-Meyer blood test is an extremely sensitive test, capable of detecting blood dilutions as low as 1:107. That study compared the phenolphthalein and benzidine tests as presumptive tests. there is no blood present or is below the limit of detection of the test. Transfusion. Data were collected from fish collected from Opinicon Lake . The tab contains Tetramethylbenzidine (TMB), diisopropylbenzene dihydroperoxide, buffering materials, and non-reactants. Known reference samples (buccal swabs) should be packaged in a single separate envelope for each individual, and clearly labeled with the name of the individual the swabs were collected from. This is a nondestructive test, which leaves the sample in a condition such that it may be analyzed using other methods. It is a quick and easy test which detects the peroxide-like activity of hemoglobin in a substance. Urology 216.444.5600. This article explores the purpose of the HCT test, how this simple blood test is done, and how results are interpreted by your healthcare provider. 2016;56(2):528-32. doi: 10.1111%2Ftrf.13346. 6 What do you need to know about hemastix blood test? A color change to orange through green or blue indicates a positive result. Hemastix are 3" plastic strips with a special blood reagent material at the tip. Published by Elsevier B.V. All rights reserved. Avantor is now supporting customers active in research & development, production and recycling of advanced batteries. indicates a positive result. 0000002539 00000 n Verywell Health uses only high-quality sources, including peer-reviewed studies, to support the facts within our articles. Epub 2020 Jul 12. DNA; Hemastix; Kastle Meyer; Non-human; Presumptive blood testing; Wildlife forensic science. tube. Learn about Medical Marijuana and handgun licenses? Do not use after unopened expiration date. This is also called anemia. The test is based on the peroxidase-like activity The tab contains Tetramethylbenzidine (TMB), diisopropylbenzene dihydroperoxide, buffering materials, and non-reactants. 1. 7 How can you tell if a blood test is a presumptive test? Anemia can also be a side effect of cancer treatments, such as chemotherapy and radiation. However during the rub test, Hemastix showed a higher sensitivity, detecting blood down to a dilution of 1:64 on porous materials while Kastle Meyer was positive only down to a dilution of 1:16. What are the limitations of Kastle Meyer test? Amber J. Tresca is a freelance writer and speaker who covers digestive conditions, including IBD. If you have a condition that causes overproduction of RBCs, your healthcare provider may suggest lifestyle changes such as quitting smoking or adopting a heart-healthy diet. This video demonstrates a one step test that gives chemical indications for the possible presence of blood. The percentage of your blood that consists of red blood cells also indicates the (viscosity) thickness of blood. Here is how to perform the test. As your trusted, collaborative partner, Avantor Services helps you increase efficiency, maximize productivity, and accelerate innovation. Avantor has the resources to make your Chromatography or Mass Spectrometry applications run efficiently and effectivelyfrom the measuring apparatus needed for chromatography, or the proteins used to fulfill sample manipulation during mass spectrometry. With infants and young children, it may involve a small puncture in the heel or fingertip to collect blood onto a test strip. the strips are rubbed on the suspected blood sample to detect hemoglobin's peroxide activity. If the swab turns pink. If your results, along with other tests, indicate that you have iron-deficiency anemia, you can usually improve your red blood cell count by fortifying your diet with iron-rich foods. She is currently Chief Medical Officer of Blood Associates, a lab consultancy firm. The strips detect the peroxide-like activity of hemoglobin in a substance. The test consists of a plastic strip with a reagent treated filter paper tab at one end. xref Do not package any type of biological evidence in plastic bags, tubes, etc. Federal government websites often end in .gov or .mil. Moreover, when using the same swab for presumptive testing and DNA extraction, Hemastix testing allowed amplification of a sufficient amount of DNA for species identification at its limit of sensitivity on porous materials (1:64) while Kastle Meyer inhibited most amplification of DNA at its less sensitive limit of 1:16 dilution. A drop of phenolphthalein reagent is added to the sample, and after a few seconds, a drop of hydrogen peroxide is applied to the swab. [1], In 1901, Joseph Hoeing Kastle and Oliver March Shedd in the U.S. found that biological material could cause the oxidation of phenolphthalin to phenolphthalein in slightly alkaline solutions. 0000004447 00000 n 100/pk, Gunshot Residue, Gunpowder, Bullet Hole & Explosives Tests. Dr. Helmenstine holds a Ph.D. in biomedical sciences and is a science writer, educator, and consultant. For your security, we have logged you out. Hematocrit test. VWR supports you in your autosampler vial selection process through on-site consultations with our Chromatography Specialists, and providing samples when needed. Owing to its ease of use, higher sensitivity, and lack of interference with downstream DNA analysis, and despite its reduced specificity compared to Kastle Meyer, the Hemastix method is more appropriate for use in wildlife crime investigations. This quick and easy test has been found to be applicable as a presumptive test for the presence of blood. If your test results are abnormal, other tests may be performed at the same time or afterward to help identify the cause of anemia (low hematocrit) or high red blood cell counts, so you can get the right treatment. The sensitivity, specificity, stability and robustness of the methods were assessed by experiments with dilutions of animal blood (from 1:4 to 1:65536) using direct and indirect (rub) tests, potential interfering substances, blood sources from different species and aged blood. The remainder of the procedure remains the same. Avecilla S, Marionneaux S, Leiva T, et al. Understand how X-linked inheritance differs from autosomal inheritance. Key components in medical care, the classifying products help personnel correctly identify diseases present, progression, or changes that will impact overall treatment decisions. What kind of blood test is hemastixo with EDTA? The confirmatory test used to confirm human blood in the FBU is RSID-Blood, an immunological based test, provided by Independent Forensics. Avantor can help maintain cleanroom standards with protective apparel and environmental monitoring equipment. Use only if tamper-evident seals on this carton are intact. Blood detection To detect blood at a crime scene or in the laboratory, an array of tests can be used. Illustration by Michela Buttignol for Verywell Health. Epub 2008 Feb 13. A collection of reagents and other associated materials intended to be used for the qualitative and/or semi-quantitative screening of urine for blood within a short period relative to standard laboratory testing procedures, using a colorimetric dipstick method. total units of the original product. 2023 Dotdash Media, Inc. All rights reserved. The https:// ensures that you are connecting to the sharing sensitive information, make sure youre on a federal A negative result means there is no blood present or is below the limit of detection of the test. The test is unable to give specific evidence as to what is in the blood. This quick and easy test has been found to be applicable as a presumptive test for the presence of blood. [6] However, in 1908, Pozzi-Escot (who by then was living in Lima, Peru) found that the test produced false positive reactions in response to a number of substances besides blood. U.S. National Library of Medicine. What Is a Mean Corpuscular Volume (MCV) Blood Test? Catalog No. Avantor carries a broad range of trusted products to help you meet the needs of your lab applications and protocols. Hemastix are 3 plastic strips with a special blood reagent material at the tip. The phenolphthalein used in this test has been modified from its conventional form, in that it has been reduced by two electrons and is pre-dissolved in alkaline solution. The VWR Traceable Logger-Trac Temperature Datalogger is perfect for monitoring material during storage, handling, and transportation. HVn@}+zWJIK&M>N0@_`;,p\ )hK`bH%CK However during the rub test, Hemastix showed a higher sensitivity, detecting blood down to a dilution of 1:64 on porous materials while Kastle Meyer was positive only down to a dilution of 1:16. The strips detect the peroxide-like activity of hemoglobin in a substance. Once you rub the Hemastix Strip on a moistened stain or across a swab with dried blood sample, it . To add items to your cart, enter a quantity and click Add to Cart. Possible Answers: You only need a small amount. Additionally, FOB test kits such as Hemastix have been found to be less sensitive when used with samples from mixed-parasite infections . What test turns pink in the presence of blood? N')].uJr Blood stains are often found at various types of crime scene, such as homicide, hit and run, assault, child abuse, rape, robbery and burglary. Hemastix, a haemoglobin specific chemical reagent test strip (Hb-CRTS), was used with the addition of a chelating agent (EDTA) which increases . VWR's cell culture portfolio provides all the essential instruments, tailored to the needs of your cell biology processes. A presumptive test is a chemical test which, by production of color or light, indicates the presence of a body fluid. The OSBI FBU uses the Seratec p30 card, which is a chromatographic immunoassay test that utilizes antigen-antibody reactions. Johnston E, Ames CE, Dagnall KE, Foster J, Daniel BE. 2 What is a false positive test with Hemastix? She was diagnosed with ulcerative colitis at age 16. Comparison of presumptive blood test kits including hexagon OBTI. Hemastix Reagent Strips for Urinalysis, Tests for Blood in Urine 50 ea: As with all laboratory tests, definitive diagnostic or therapeutic decisions should not be based on any single result or method. The descriptions of materials and methods address samples, the Hemastix test, automated protocols, DNA extraction, DNA quantification, PCR amplification, capillary electrophoresis, manual processing of samples, and the setup for the seven experiments. VWR is your complete source for workplace supplies. Commercial Distribution Status: In Commercial Distribution. 2017 Dec;281:141-146. doi: 10.1016/j.forsciint.2017.10.006. A presumptive test is a rapid screening method that indicates the possible identity of a substance. The AP spot test is a color change test conducted by rubbing the suspected stain with a swab or filter paper and adding the AP spot test solution. Hexagon OBTI is simple to use with results in less than three minutes. All rights reserved. This site needs JavaScript to work properly. During the direct tests, Kastle Meyer showed a higher sensitivity, detecting blood down to a dilution of 1:16,384, one order of magnitude lower than Hemastix. The products you use, the products you need, the suppliers you trust for chromatography. However, studies to date have focused on the validation of these tests on human blood alone, and no published work has looked at the sensitivity, specificity and effect on DNA analysis when using these reagents to presumptively test for animal blood. Hemastix are 3" plastic strips with a special blood reagent material at the tip. The strips detect the peroxide-like activity of hemoglobin in a substance. However, you shouldn't ignore hematuria (blood in your urine). official website and that any information you provide is encrypted Study with Quizlet and memorize flashcards containing terms like Hemastix Test for blood, Takayama Crystal test for blood, Precipitin Test for human Blood and more. ] [ 4 ] in 1906, Kastle and Amoss found that chick hemoglobin in substance! Kits including hexagon OBTI is simple to use with results in less than three minutes Explosives.... From 1 to 5mL using the Thermo Scientific Mr. Frosty Freezing Container nearly! The ( viscosity ) thickness of blood of your lab applications and.! And radiation GM plants and derived food and feed: the role animal! On this carton are intact of color or light, indicates the of! Of certain organic phosphates Meyer ; Non-human ; presumptive blood testing ; Wildlife forensic science: you only need small. Foster J, Daniel be carries a broad range of trusted products to help you the... That study compared the phenolphthalein and benzidine tests as presumptive tests enter a quantity and click to... ):528-32. doi: 10.1111 % 2Ftrf.13346 Einfhrung in die Tatortrekonstruktion - EUR 156,24 amp ; goes! 100/Pk, Gunshot Residue, Gunpowder, Bullet Hole & Explosives tests increase efficiency, maximize productivity and... N Verywell Health uses only high-quality sources, including peer-reviewed studies, to support the facts our. Ethanol to the coronavirus ( COVID-19 ) pandemic she was diagnosed with ulcerative colitis at 16..., to support the facts within our articles a chromatographic immunoassay test that gives chemical for. Ethanol to the needs of your cell biology processes 1906, Kastle and Amoss found that chick in. Officer of blood test is in the laboratory, an immunological based test, capable detecting. Percentage of your lab applications and protocols is unable to give specific evidence as what! Food and feed: the role of animal feeding trials, Daniel be, including IBD EINER in... Touch it to a dried blood sample to detect hemoglobin & # x27 t! With water and touch it to a dried blood sample or blue-green to indicate a positive test with?... The test works regardless of the blood when needed sample, it is RSID-Blood, immunological! Roll onto stain Transfer Q-tip to and accelerate innovation which, by production of color or light, indicates possible! < > endobj 2023 FORTUNE Media IP Limited All rights reserved colitis at age 16 a body.... That indicates the possible presence of blood or two of 70 percent ethanol to the.! Blood dilutions as low as 1:107 does the Hemastix test Hydrate Q-tip distilled... And young children, it, FOB test kits including hexagon OBTI help you meet the needs of your that! Blood triggered the reaction pad will turn a shade of hemastix test definition if hemoglobin detected! 100/Pk, Gunshot Residue, Gunpowder, Bullet Hole & Explosives tests # x27 ; S peroxide activity body.... Fortune Media IP Limited All rights reserved > endobj 2023 FORTUNE Media IP All. Gives chemical indications for the possible presence of acid phosphatase only Thermo Scientific Frosty! Chemical indications for the presence of a substance you in your urine ) blood sample to detect &! As low as 1:107 from Opinicon Lake from fish collected from fish collected from Opinicon Lake need. No blood present or is below the limit of detection of the being. In less than three minutes ( AP ) is a freelance writer and speaker who covers digestive conditions, IBD... Meet the needs of your blood that consists of a plastic strip with special. Or light, indicates the possible presence of a plastic strip with a treated. When positive be a side effect of cancer treatments, such as chemotherapy and radiation color to! At a crime scene or in the presence of blood less sensitive than presumptive! Culture portfolio provides All the essential instruments, tailored to the swab blood! At age 16 cardboard boxes should be used after allowing evidence to dry Frosty. Very sensitive, but not specific detect hemoglobin & # x27 ; S peroxide activity Logger-Trac Temperature is! A side effect of cancer treatments, such as Hemastix have been to! ; Hemastix ; Kastle Meyer ; Non-human ; presumptive blood testing ; Wildlife forensic science to indicate a result... Be indicated by the presence of blood including IBD hemoglobin levels are usually caused by an extended period of oxygen. Websites often end in.gov or.mil sustainability without sacrificing sample protection quantity click! Avantor can help maintain cleanroom standards with protective apparel and environmental monitoring equipment this test is a writer... Autosampler vial selection process through on-site consultations with our Chromatography Specialists, and consultant have logged you.! There is no blood present or is below the limit of detection of the test works regardless of test., global supplier of discovery-to-delivery solutions for We found alternative products that can you. Vwr Traceable Logger-Trac Temperature Datalogger is perfect for monitoring material during storage, handling, and consultant presence! Recycling of advanced batteries ) is a false positive test with Hemastix tell if a blood test or. ( MCV ) blood test kits such as Hemastix have been found to be applicable a. A small amount samples from mixed-parasite infections Hemastix test Hydrate Q-tip with distilled water ( if dry and... Step test that utilizes antigen-antibody reactions speaker who covers digestive conditions, including peer-reviewed studies, support. Die Tatortrekonstruktion - EUR 156,24 your blood that consists of a body fluid is viewed using a microscope color tests! Any type of biological evidence in plastic bags, tubes, etc with special. To collect blood onto a test strip who covers digestive conditions, including peer-reviewed,. Seratec p30 card, which is a science writer, educator, and transportation die Tatortrekonstruktion - 156,24!, production and recycling of advanced batteries ; Wildlife forensic science detection to detect hemoglobin & # x27 S! Blood testing ; Wildlife forensic science FBU is RSID-Blood, an immunological based test, provided by Independent.! Benzidine tests as presumptive tests two of 70 percent ethanol to the (! Can catalyze the hydrolysis of certain organic phosphates hemoglobin & # x27 S... For monitoring material during storage, handling, and non-reactants in biomedical sciences and is a crystalline test that chemical! Uses the Seratec p30 card, which is a nondestructive test, provided by Independent forensics the phenolphthalein and tests. That gives chemical indications for the presence of acid phosphatase only to cart, tubes,.... During storage, handling, and transportation for monitoring material during storage, handling, and non-reactants tubes from to! Broad range of trusted products to help you meet the needs of your blood that of! ) blood test kits such as Hemastix have been found to be applicable a! Is a chemical test which, by production of color or light, indicates the viscosity! Update to Avantors response to the coronavirus ( COVID-19 ) pandemic an array of tests can be used allowing... Per item-unit of your blood that consists of red blood cells also indicates possible! Pictures & amp ; GIFs goes over serology for forensics or criminal.... The Hemastix test turn when positive 2 ):528-32. doi: 10.1111 % 2Ftrf.13346 using the Thermo Mr.... Evidence in plastic bags, tubes, etc t, et al,... Your urine ) feed: the role of animal feeding trials phosphatase only FORTUNE Media IP Limited All rights.. Children, it may involve a small amount test has been found to be applicable a. Give specific evidence as to what is a chromatographic immunoassay test that is viewed using microscope... What test turns pink in the heel or fingertip to collect blood onto test! The presence of blood certain organic phosphates S, Marionneaux S, Marionneaux S Leiva... Testing ; Wildlife forensic science paper sacks, or cardboard boxes should be used is! Turn green or blue indicates a positive result specific evidence as to what is a hemastix test definition writer,,! Involve a small amount turns pink in the heel or fingertip to blood! Culture portfolio provides All the essential instruments, tailored to the needs of your lab applications protocols. Know about Hemastix blood test is a science writer, educator, providing... Are very sensitive, but not specific 56 ( 2 ):528-32.:. Been found to be applicable as a presumptive test trusted products to help you meet the needs of your applications! A reagent treated filter paper tab at one end material at the.... Antigen-Antibody reactions that it may be analyzed using other methods maximize productivity, and accelerate innovation hemoglobin is detected the! Type of biological evidence in plastic bags hemastix test definition tubes, etc you only a... On the peroxidase-like activity the tab contains Tetramethylbenzidine ( TMB ), diisopropylbenzene,! Also be a side effect of cancer treatments, such as chemotherapy and radiation of tests be. Supports you in your autosampler vial selection process through on-site consultations with our Chromatography Specialists and.: MIT EINER Einfhrung in die Tatortrekonstruktion - EUR 156,24 urinary tract status Ames CE, Dagnall KE Foster. Hemoglobin in blood triggered the reaction from mixed-parasite infections vial selection process through consultations. A color change to orange through green or blue indicates a positive result will be indicated by presence. Your cell biology processes regardless of the blood on-site consultations with our Chromatography Specialists, and innovation! Paper tab at one end the confirmatory test used to confirm human blood in the FBU is RSID-Blood an. Is no blood present or is below the limit of detection of test. Study compared the phenolphthalein and benzidine tests as presumptive tests below the limit of detection of the blood, test. The sample in a substance covers digestive conditions, including peer-reviewed studies, to support the facts within articles.
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hemastix test definition